AB0029 THE METABOLIC HIERARCHY OF IMMUNE PROCESSES IN HUMAN MONOCYTES

Background: At sites of inflammation, monocytes carry out specific immunological functions while facing challenging bioenergetic restrictions. Objectives: Here, we investigated the potential human to adapt under conditions reduced energy supply by gradually inhibiting oxidative phosphorylation (OXPHOS) glucose free conditions. Methods: We modelled this with myxothiazol, an inhibitor mitochondrial respiration, at 0, 2 and 4 pmol/10 6 cells decrease ATP production for 0%, 25% 66% For three levels, assessed (i) phagocytosis FITC-labelled E.coli using flow cytometry, reactive oxygen species (ROS) through NADPH oxidase (NOX) as determined VAS2870-sensitive OCR a Clark-type electrode, (iii) generation steady state level electrode luminometric assessment (iv) expression surface activation markers CD16, CD80, CD11b, HLA-DR (v) inflammatory cytokines IL-1β, IL-6 TNF-α cytometry in peripheral blood-derived without LPS-stimulation. Results: As prerequisite our study, demonstrate that survived strong inhibition respiration any sign apoptosis cytometry. result OXPHOS, reduction (ROS NOX), ATPase-dependent steady-state levels. Focusing on immune function, observed were least sensitive HLA-DR, IL-1β most affected OXPHOS. Conclusion: Our data energy-dependent hierarchy monocytes, which may represent therapeutic target monocyte-mediated diseases. Disclosure Interests: None declared